Spray-on Bandage for Monitoring of Tissue Oxygenation
Pieter G.L. Koolen, M.D., Ahmed M.S. Ibrahim, M.D., Kuylhee Kim, M.D., Zongxi Li, PhD, Emmanuel Roussakis, PhD, Conor Evans, PhD, Samuel J. Lin, M.D..
Beth Israel Deaconess Medical Center, Boston, MA, USA.
Punch biopsy with histologic analysis is still the gold standard for assessment of wound depth in burns. Recently, less invasive optical techniques have been developed, including laser Doppler imaging (LDI), indocyanine green videoangiography (ICG), and near infrared spectroscopy (NIRS). However, these are bulky, expensive, and require significant training. We aim to develop an interactive wound dressing using optical oxygen sensors without the need for dressing removal.
Proof-of- concept studies were performed by direct application of oxygen sensing films to porcine burn wounds. The sensing films were prepared from a solution of porphyrins, an oxygen- insensitive reference fluorophore (coumarin 500), and a polymer dissolved in an alcohol- based solvent that can quickly and easily be applied to surfaces by spraying.
Upon application to surfaces, the alcohol rapidly evaporated leaving behind a flexible polymer film into which the sensor molecules are tightly enmeshed along with green fluorescent molecules. The oxygen content within the bandage equalizes with tissue oxygen, and can be quantified by the red to green channel intensity ratio captured by the imaging device. A two dimensional colorimetric map is generated that reflects the equilibrium tissue oxygenation (Figure 1).
Proof-of-concept studies demonstrated the capacity of these oxygen sensing films to accurately detect underlying tissue oxygenation and provide a means of assessing wound severity.
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